Construct: shRNA TRCN0000421227

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002045.3-1302s21c1
DNA Barcode:
GTCAAACAGTGTGGCTTAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAP43 (2596)
Gene Description:
growth associated protein 43
Transcript:
RefSeq NM_002045.3 (NON-CURRENT)
Match location:
Position 1302 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421227)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAAACAGTGTGGCTTAAAC
Hairpin Sequence:
5'-CCGG-GTCAAACAGTGTGGCTTAAAC-CTCGAG-GTTTAAGCCACACTGTTTGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAAACAGTGTGGCTTAAACCTCGAGGTTTAAGCCACACTGTTTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAAACAGTGTGGCTTAAACCTCGAGGTTTAAGCCACACTGTTTGAC-3'

Other clones with same target sequence:

(none)