Construct: shRNA TRCN0000421238

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001042690.1-1000s21c1
DNA Barcode:
ATCACCAAGTCCAGCGTCTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MSANTD1 (345222)
Gene Description:
Myb/SANT DNA binding domain containing 1
Transcript:
RefSeq NM_001042690.1 (NON-CURRENT)
Match location:
Position 1000 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421238)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCACCAAGTCCAGCGTCTAG
Hairpin Sequence:
5'-CCGG-ATCACCAAGTCCAGCGTCTAG-CTCGAG-CTAGACGCTGGACTTGGTGAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCACCAAGTCCAGCGTCTAGCTCGAGCTAGACGCTGGACTTGGTGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCACCAAGTCCAGCGTCTAGCTCGAGCTAGACGCTGGACTTGGTGAT-3'

Other clones with same target sequence:

(none)