Construct: shRNA TRCN0000421291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152277.2-275s21c1
DNA Barcode:
TACGCAGCAAGAGGGATGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBTD2 (92181)
Gene Description:
ubiquitin domain containing 2
Transcript:
RefSeq NM_152277.2 (NON-CURRENT)
Match location:
Position 275 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACGCAGCAAGAGGGATGAAT
Hairpin Sequence:
5'-CCGG-TACGCAGCAAGAGGGATGAAT-CTCGAG-ATTCATCCCTCTTGCTGCGTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACGCAGCAAGAGGGATGAATCTCGAGATTCATCCCTCTTGCTGCGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACGCAGCAAGAGGGATGAATCTCGAGATTCATCCCTCTTGCTGCGTA-3'

Other clones with same target sequence:

(none)