Construct: shRNA TRCN0000421324

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081366.1-4794s21c1
DNA Barcode:
TCATTTCGCTCAGAATATTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps8 (209018)
Gene Description:
VPS8 CORVET complex subunit
Transcript:
RefSeq NM_001081366.1 (NON-CURRENT)
Match location:
Position 4794 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421324)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATTTCGCTCAGAATATTAC
Hairpin Sequence:
5'-CCGG-TCATTTCGCTCAGAATATTAC-CTCGAG-GTAATATTCTGAGCGAAATGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATTTCGCTCAGAATATTACCTCGAGGTAATATTCTGAGCGAAATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATTTCGCTCAGAATATTACCTCGAGGTAATATTCTGAGCGAAATGA-3'

Other clones with same target sequence:

(none)