Construct: shRNA TRCN0000421352
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_138389.2-2247s21c1
- DNA Barcode:
- CCTGGTTAAGCATGCTATATT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FAM114A1 (92689)
- Gene Description:
- family with sequence similarity 114 member A1
- Transcript:
- RefSeq NM_138389.2 (NON-CURRENT)
- Match location:
- Position 2247 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000421352)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTGGTTAAGCATGCTATATT
- Hairpin Sequence:
-
5'-CCGG-CCTGGTTAAGCATGCTATATT-CTCGAG-AATATAGCATGCTTAACCAGG-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTGGTTAAGCATGCTATATTCTCGAGAATATAGCATGCTTAACCAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTGGTTAAGCATGCTATATTCTCGAGAATATAGCATGCTTAACCAGG-3'
Other clones with same target sequence:
(none)
