Construct: shRNA TRCN0000421358

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010459.6-1284s21c1
DNA Barcode:
CCTCGACACTTGGCTAACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hoxb4 (15412)
Gene Description:
homeobox B4
Transcript:
RefSeq NM_010459.6 (NON-CURRENT)
Match location:
Position 1284 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421358)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGACACTTGGCTAACAAA
Hairpin Sequence:
5'-CCGG-CCTCGACACTTGGCTAACAAA-CTCGAG-TTTGTTAGCCAAGTGTCGAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGACACTTGGCTAACAAACTCGAGTTTGTTAGCCAAGTGTCGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGACACTTGGCTAACAAACTCGAGTTTGTTAGCCAAGTGTCGAGG-3'

Other clones with same target sequence:

(none)