Construct: shRNA TRCN0000421372

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178821.1-1494s21c1
DNA Barcode:
AGTTATGCCACTCCAATATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAW1 (164781)
Gene Description:
dynein assembly factor with WD repeats 1
Transcript:
RefSeq NM_178821.1 (NON-CURRENT)
Match location:
Position 1494 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421372)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTATGCCACTCCAATATTA
Hairpin Sequence:
5'-CCGG-AGTTATGCCACTCCAATATTA-CTCGAG-TAATATTGGAGTGGCATAACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTATGCCACTCCAATATTACTCGAGTAATATTGGAGTGGCATAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTATGCCACTCCAATATTACTCGAGTAATATTGGAGTGGCATAACT-3'

Other clones with same target sequence:

(none)