Construct: shRNA TRCN0000421410

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175013.2-1254s21c1
DNA Barcode:
TTGGTCTGGCTGTCCATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pgm5 (226041)
Gene Description:
phosphoglucomutase 5
Transcript:
RefSeq NM_175013.2 (CURRENT)
Match location:
Position 1254 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421410)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGTCTGGCTGTCCATTATT
Hairpin Sequence:
5'-CCGG-TTGGTCTGGCTGTCCATTATT-CTCGAG-AATAATGGACAGCCAGACCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGTCTGGCTGTCCATTATTCTCGAGAATAATGGACAGCCAGACCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGTCTGGCTGTCCATTATTCTCGAGAATAATGGACAGCCAGACCAA-3'

Other clones with same target sequence:

(none)