Construct: shRNA TRCN0000421425

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005946.2-286s21c1
DNA Barcode:
TAGAAAGAGTGACCTGCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MT1A (4489)
Gene Description:
metallothionein 1A
Transcript:
RefSeq NM_005946.2 (NON-CURRENT)
Match location:
Position 286 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421425)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGAAAGAGTGACCTGCACAA
Hairpin Sequence:
5'-CCGG-TAGAAAGAGTGACCTGCACAA-CTCGAG-TTGTGCAGGTCACTCTTTCTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGAAAGAGTGACCTGCACAACTCGAGTTGTGCAGGTCACTCTTTCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGAAAGAGTGACCTGCACAACTCGAGTTGTGCAGGTCACTCTTTCTA-3'

Other clones with same target sequence:

(none)