Construct: shRNA TRCN0000421429

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002925.3-114s21c1
DNA Barcode:
CCTCAAGAGCACAGCCAAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGS10 (6001)
Gene Description:
regulator of G protein signaling 10
Transcript:
RefSeq NM_002925.3 (CURRENT)
Match location:
Position 114 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421429)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAAGAGCACAGCCAAATG
Hairpin Sequence:
5'-CCGG-CCTCAAGAGCACAGCCAAATG-CTCGAG-CATTTGGCTGTGCTCTTGAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAAGAGCACAGCCAAATGCTCGAGCATTTGGCTGTGCTCTTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAAGAGCACAGCCAAATGCTCGAGCATTTGGCTGTGCTCTTGAGG-3'

Other clones with same target sequence:

(none)