Construct: shRNA TRCN0000421434

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006796.1-1272s21c1
DNA Barcode:
GCTAGAGTCCGAGACTTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AFG3L2 (10939)
Gene Description:
AFG3 like matrix AAA peptidase subunit 2
Transcript:
RefSeq NM_006796.1 (NON-CURRENT)
Match location:
Position 1272 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421434)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGAGTCCGAGACTTATTT
Hairpin Sequence:
5'-CCGG-GCTAGAGTCCGAGACTTATTT-CTCGAG-AAATAAGTCTCGGACTCTAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGAGTCCGAGACTTATTTCTCGAGAAATAAGTCTCGGACTCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGAGTCCGAGACTTATTTCTCGAGAAATAAGTCTCGGACTCTAGC-3'

Other clones with same target sequence:

(none)