Construct: shRNA TRCN0000421542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004776.3-677s21c1
DNA Barcode:
GTGGAAGGTGGCGATCCTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
B4GALT5 (9334)
Gene Description:
beta-1,4-galactosyltransferase 5
Transcript:
RefSeq NM_004776.3 (NON-CURRENT)
Match location:
Position 677 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGAAGGTGGCGATCCTTAT
Hairpin Sequence:
5'-CCGG-GTGGAAGGTGGCGATCCTTAT-CTCGAG-ATAAGGATCGCCACCTTCCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGAAGGTGGCGATCCTTATCTCGAGATAAGGATCGCCACCTTCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGAAGGTGGCGATCCTTATCTCGAGATAAGGATCGCCACCTTCCAC-3'

Other clones with same target sequence:

(none)