Construct: shRNA TRCN0000421549

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005472.4-887s21c1
DNA Barcode:
AGCCCATGAACAAACATATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNE3 (10008)
Gene Description:
potassium voltage-gated channel subfamily E regulatory subunit 3
Transcript:
RefSeq NM_005472.4 (CURRENT)
Match location:
Position 887 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421549)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCCATGAACAAACATATAA
Hairpin Sequence:
5'-CCGG-AGCCCATGAACAAACATATAA-CTCGAG-TTATATGTTTGTTCATGGGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCCATGAACAAACATATAACTCGAGTTATATGTTTGTTCATGGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCCATGAACAAACATATAACTCGAGTTATATGTTTGTTCATGGGCT-3'

Other clones with same target sequence:

(none)