Construct: shRNA TRCN0000421554

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007341.2-892s21c1
DNA Barcode:
CCAGGGTTACTGAGGACTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3BGR (6450)
Gene Description:
SH3 domain binding glutamate rich protein
Transcript:
RefSeq NM_007341.2 (CURRENT)
Match location:
Position 892 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421554)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGGTTACTGAGGACTTAT
Hairpin Sequence:
5'-CCGG-CCAGGGTTACTGAGGACTTAT-CTCGAG-ATAAGTCCTCAGTAACCCTGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGGTTACTGAGGACTTATCTCGAGATAAGTCCTCAGTAACCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGGTTACTGAGGACTTATCTCGAGATAAGTCCTCAGTAACCCTGG-3'

Other clones with same target sequence:

(none)