Construct: shRNA TRCN0000421636

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080485.2-1678s21c1
DNA Barcode:
GAAGTACCATCAAGGTATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF275 (10838)
Gene Description:
zinc finger protein 275
Transcript:
RefSeq NM_001080485.2 (NON-CURRENT)
Match location:
Position 1678 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421636)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTACCATCAAGGTATTTC
Hairpin Sequence:
5'-CCGG-GAAGTACCATCAAGGTATTTC-CTCGAG-GAAATACCTTGATGGTACTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTACCATCAAGGTATTTCCTCGAGGAAATACCTTGATGGTACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTACCATCAAGGTATTTCCTCGAGGAAATACCTTGATGGTACTTC-3'

Other clones with same target sequence:

(none)