Construct: shRNA TRCN0000421667

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002728.4-441s21c1
DNA Barcode:
TTTACTTGCCGGAGGTGCTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRG2 (5553)
Gene Description:
proteoglycan 2, pro eosinophil major basic protein
Transcript:
RefSeq NM_002728.4 (NON-CURRENT)
Match location:
Position 441 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421667)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTACTTGCCGGAGGTGCTAC
Hairpin Sequence:
5'-CCGG-TTTACTTGCCGGAGGTGCTAC-CTCGAG-GTAGCACCTCCGGCAAGTAAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTACTTGCCGGAGGTGCTACCTCGAGGTAGCACCTCCGGCAAGTAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTACTTGCCGGAGGTGCTACCTCGAGGTAGCACCTCCGGCAAGTAAA-3'

Other clones with same target sequence:

(none)