Construct: shRNA TRCN0000421701

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_147129.2-1080s21c1
DNA Barcode:
ACTGCCGCTGCGCAGAATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALS2CL (259173)
Gene Description:
ALS2 C-terminal like
Transcript:
RefSeq NM_147129.2 (NON-CURRENT)
Match location:
Position 1080 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421701)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGCCGCTGCGCAGAATATA
Hairpin Sequence:
5'-CCGG-ACTGCCGCTGCGCAGAATATA-CTCGAG-TATATTCTGCGCAGCGGCAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGCCGCTGCGCAGAATATACTCGAGTATATTCTGCGCAGCGGCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGCCGCTGCGCAGAATATACTCGAGTATATTCTGCGCAGCGGCAGT-3'

Other clones with same target sequence:

(none)