Construct: shRNA TRCN0000421737

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015993.2-559s21c1
DNA Barcode:
GCCTCGTTCTTTGCGTGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLLP (51090)
Gene Description:
plasmolipin
Transcript:
RefSeq NM_015993.2 (NON-CURRENT)
Match location:
Position 559 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421737)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCGTTCTTTGCGTGTTTG
Hairpin Sequence:
5'-CCGG-GCCTCGTTCTTTGCGTGTTTG-CTCGAG-CAAACACGCAAAGAACGAGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCGTTCTTTGCGTGTTTGCTCGAGCAAACACGCAAAGAACGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCGTTCTTTGCGTGTTTGCTCGAGCAAACACGCAAAGAACGAGGC-3'

Other clones with same target sequence:

(none)