Construct: shRNA TRCN0000421741

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145533.1-542s21c1
DNA Barcode:
ATTTATACAACGAGGTCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smox (228608)
Gene Description:
spermine oxidase
Transcript:
RefSeq NM_145533.1 (NON-CURRENT)
Match location:
Position 542 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421741)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTTATACAACGAGGTCTATA
Hairpin Sequence:
5'-CCGG-ATTTATACAACGAGGTCTATA-CTCGAG-TATAGACCTCGTTGTATAAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTTATACAACGAGGTCTATACTCGAGTATAGACCTCGTTGTATAAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTTATACAACGAGGTCTATACTCGAGTATAGACCTCGTTGTATAAAT-3'

Other clones with same target sequence:

  1. TRCN0000359567