Construct: shRNA TRCN0000421795

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173815.4-1105s21c1
DNA Barcode:
CAATTGGCTCTTGCCTTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CES4A (283848)
Gene Description:
carboxylesterase 4A
Transcript:
RefSeq NM_173815.4 (NON-CURRENT)
Match location:
Position 1105 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421795)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATTGGCTCTTGCCTTATAA
Hairpin Sequence:
5'-CCGG-CAATTGGCTCTTGCCTTATAA-CTCGAG-TTATAAGGCAAGAGCCAATTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATTGGCTCTTGCCTTATAACTCGAGTTATAAGGCAAGAGCCAATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATTGGCTCTTGCCTTATAACTCGAGTTATAAGGCAAGAGCCAATTG-3'

Other clones with same target sequence:

(none)