Construct: shRNA TRCN0000421817

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010129.2-510s21c1
DNA Barcode:
TCTTCTACGCTACCGGCCTCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Emp3 (13732)
Gene Description:
epithelial membrane protein 3
Transcript:
RefSeq NM_010129.2 (CURRENT)
Match location:
Position 510 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421817)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTCTACGCTACCGGCCTCT
Hairpin Sequence:
5'-CCGG-TCTTCTACGCTACCGGCCTCT-CTCGAG-AGAGGCCGGTAGCGTAGAAGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTCTACGCTACCGGCCTCTCTCGAGAGAGGCCGGTAGCGTAGAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTCTACGCTACCGGCCTCTCTCGAGAGAGGCCGGTAGCGTAGAAGA-3'

Other clones with same target sequence:

(none)