Construct: shRNA TRCN0000421852
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_014513.2-1297s21c1
- DNA Barcode:
- TCCTTTGCTTAGCCCACAATT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KIR2DS5 (3810)
- Gene Description:
- killer cell immunoglobulin like receptor, two Ig domains and short cytoplasmic tail 5
- Transcript:
- RefSeq NM_014513.2 (CURRENT)
- Match location:
- Position 1297 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000421852)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TCCTTTGCTTAGCCCACAATT
- Hairpin Sequence:
-
5'-CCGG-TCCTTTGCTTAGCCCACAATT-CTCGAG-AATTGTGGGCTAAGCAAAGGA-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTCCTTTGCTTAGCCCACAATTCTCGAGAATTGTGGGCTAAGCAAAGGATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATCCTTTGCTTAGCCCACAATTCTCGAGAATTGTGGGCTAAGCAAAGGA-3'
Other clones with same target sequence:
(none)
