Construct: shRNA TRCN0000421886

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177602.3-960s21c1
DNA Barcode:
TCAGTGGTGGCTTCGACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr25 (212198)
Gene Description:
WD repeat domain 25
Transcript:
RefSeq NM_177602.3 (CURRENT)
Match location:
Position 960 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421886)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAGTGGTGGCTTCGACTTTG
Hairpin Sequence:
5'-CCGG-TCAGTGGTGGCTTCGACTTTG-CTCGAG-CAAAGTCGAAGCCACCACTGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAGTGGTGGCTTCGACTTTGCTCGAGCAAAGTCGAAGCCACCACTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAGTGGTGGCTTCGACTTTGCTCGAGCAAAGTCGAAGCCACCACTGA-3'

Other clones with same target sequence:

(none)