Construct: shRNA TRCN0000421920

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175114.3-2002s21c1
DNA Barcode:
TGTACTGCCTTAACGACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trak1 (67095)
Gene Description:
trafficking protein, kinesin binding 1
Transcript:
RefSeq NM_175114.3 (CURRENT)
Match location:
Position 2002 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421920)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTACTGCCTTAACGACTTTG
Hairpin Sequence:
5'-CCGG-TGTACTGCCTTAACGACTTTG-CTCGAG-CAAAGTCGTTAAGGCAGTACA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTACTGCCTTAACGACTTTGCTCGAGCAAAGTCGTTAAGGCAGTACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTACTGCCTTAACGACTTTGCTCGAGCAAAGTCGTTAAGGCAGTACA-3'

Other clones with same target sequence:

  1. TRCN0000294179