Construct: shRNA TRCN0000422052
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_018708.2-2347s21c1
- DNA Barcode:
- GTGCTAAGAGGACAGTCTTTC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FEM1A (55527)
- Gene Description:
- fem-1 homolog A
- Transcript:
- RefSeq NM_018708.2 (NON-CURRENT)
- Match location:
- Position 2347 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000422052)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTGCTAAGAGGACAGTCTTTC
- Hairpin Sequence:
-
5'-CCGG-GTGCTAAGAGGACAGTCTTTC-CTCGAG-GAAAGACTGTCCTCTTAGCAC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTGCTAAGAGGACAGTCTTTCCTCGAGGAAAGACTGTCCTCTTAGCACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTGCTAAGAGGACAGTCTTTCCTCGAGGAAAGACTGTCCTCTTAGCAC-3'
Other clones with same target sequence:
(none)
