Construct: shRNA TRCN0000422080

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004706.3-1897s21c1
DNA Barcode:
GGAAATTCTACACCACGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGEF1 (9138)
Gene Description:
Rho guanine nucleotide exchange factor 1
Transcript:
RefSeq NM_004706.3 (NON-CURRENT)
Match location:
Position 1897 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422080)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAAATTCTACACCACGTCAA
Hairpin Sequence:
5'-CCGG-GGAAATTCTACACCACGTCAA-CTCGAG-TTGACGTGGTGTAGAATTTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAAATTCTACACCACGTCAACTCGAGTTGACGTGGTGTAGAATTTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAAATTCTACACCACGTCAACTCGAGTTGACGTGGTGTAGAATTTCC-3'

Other clones with same target sequence:

(none)