Construct: shRNA TRCN0000422099

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020394.3-1148s21c1
DNA Barcode:
TTGTTGTCATACCTTACTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF695 (57116)
Gene Description:
zinc finger protein 695
Transcript:
RefSeq NM_020394.3 (NON-CURRENT)
Match location:
Position 1148 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422099)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTTGTCATACCTTACTCAA
Hairpin Sequence:
5'-CCGG-TTGTTGTCATACCTTACTCAA-CTCGAG-TTGAGTAAGGTATGACAACAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTTGTCATACCTTACTCAACTCGAGTTGAGTAAGGTATGACAACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTTGTCATACCTTACTCAACTCGAGTTGAGTAAGGTATGACAACAA-3'

Other clones with same target sequence:

(none)