Construct: shRNA TRCN0000422102

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183389.1-824s21c1
DNA Barcode:
CAAAGTTCCAGACTGATATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Duxbl1 (278672)
Gene Description:
double homeobox B-like 1
Transcript:
RefSeq NM_183389.1 (CURRENT)
Match location:
Position 824 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422102)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGTTCCAGACTGATATTC
Hairpin Sequence:
5'-CCGG-CAAAGTTCCAGACTGATATTC-CTCGAG-GAATATCAGTCTGGAACTTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGTTCCAGACTGATATTCCTCGAGGAATATCAGTCTGGAACTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGTTCCAGACTGATATTCCTCGAGGAATATCAGTCTGGAACTTTG-3'

Other clones with same target sequence:

(none)