Construct: shRNA TRCN0000422108

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080833.2-266s21c1
DNA Barcode:
GAACAAGCTTCTGGAACTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBBP8NL (140893)
Gene Description:
RBBP8 N-terminal like
Transcript:
RefSeq NM_080833.2 (NON-CURRENT)
Match location:
Position 266 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422108)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAAGCTTCTGGAACTGAA
Hairpin Sequence:
5'-CCGG-GAACAAGCTTCTGGAACTGAA-CTCGAG-TTCAGTTCCAGAAGCTTGTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAAGCTTCTGGAACTGAACTCGAGTTCAGTTCCAGAAGCTTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAAGCTTCTGGAACTGAACTCGAGTTCAGTTCCAGAAGCTTGTTC-3'

Other clones with same target sequence:

(none)