Construct: shRNA TRCN0000422109

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003510.2-75s21c1
DNA Barcode:
GTTCCCAGTGGGCCGAGTGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2AC15 (8330)
Gene Description:
H2A clustered histone 15
Transcript:
RefSeq NM_003510.2 (CURRENT)
Match location:
Position 75 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422109)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCCCAGTGGGCCGAGTGCA
Hairpin Sequence:
5'-CCGG-GTTCCCAGTGGGCCGAGTGCA-CTCGAG-TGCACTCGGCCCACTGGGAAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCCCAGTGGGCCGAGTGCACTCGAGTGCACTCGGCCCACTGGGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCCCAGTGGGCCGAGTGCACTCGAGTGCACTCGGCCCACTGGGAAC-3'

Other clones with same target sequence:

(none)