Construct: shRNA TRCN0000422120

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012232.5-1106s21c1
DNA Barcode:
GACAAGTTGCGCAAATCCTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAVIN1 (284119)
Gene Description:
caveolae associated protein 1
Transcript:
RefSeq NM_012232.5 (NON-CURRENT)
Match location:
Position 1106 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422120)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAAGTTGCGCAAATCCTTC
Hairpin Sequence:
5'-CCGG-GACAAGTTGCGCAAATCCTTC-CTCGAG-GAAGGATTTGCGCAACTTGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAAGTTGCGCAAATCCTTCCTCGAGGAAGGATTTGCGCAACTTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAAGTTGCGCAAATCCTTCCTCGAGGAAGGATTTGCGCAACTTGTC-3'

Other clones with same target sequence:

(none)