Construct: shRNA TRCN0000422233

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017489.2-659s21c1
DNA Barcode:
AGCAAATTGCTTATGATAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TERF1 (7013)
Gene Description:
telomeric repeat binding factor 1
Transcript:
RefSeq NM_017489.2 (NON-CURRENT)
Match location:
Position 659 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422233)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAAATTGCTTATGATAATC
Hairpin Sequence:
5'-CCGG-AGCAAATTGCTTATGATAATC-CTCGAG-GATTATCATAAGCAATTTGCT-TTTTTTTGAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAAATTGCTTATGATAATCCTCGAGGATTATCATAAGCAATTTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAAATTGCTTATGATAATCCTCGAGGATTATCATAAGCAATTTGCT-3'

Other clones with same target sequence:

(none)