Construct: shRNA TRCN0000422307

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017631.5-1083s21c1
DNA Barcode:
GTGTTACTTCATGCTCATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDX60 (55601)
Gene Description:
DExD/H-box helicase 60
Transcript:
RefSeq NM_017631.5 (NON-CURRENT)
Match location:
Position 1083 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422307)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTTACTTCATGCTCATTAT
Hairpin Sequence:
5'-CCGG-GTGTTACTTCATGCTCATTAT-CTCGAG-ATAATGAGCATGAAGTAACAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTTACTTCATGCTCATTATCTCGAGATAATGAGCATGAAGTAACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTTACTTCATGCTCATTATCTCGAGATAATGAGCATGAAGTAACAC-3'

Other clones with same target sequence:

(none)