Construct: shRNA TRCN0000422336

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173471.3-1271s21c1
DNA Barcode:
GTCAGCAGTCTGTGGAGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC25A26 (115286)
Gene Description:
solute carrier family 25 member 26
Transcript:
RefSeq NM_173471.3 (CURRENT)
Match location:
Position 1271 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422336)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGCAGTCTGTGGAGCTTT
Hairpin Sequence:
5'-CCGG-GTCAGCAGTCTGTGGAGCTTT-CTCGAG-AAAGCTCCACAGACTGCTGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGCAGTCTGTGGAGCTTTCTCGAGAAAGCTCCACAGACTGCTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGCAGTCTGTGGAGCTTTCTCGAGAAAGCTCCACAGACTGCTGAC-3'

Other clones with same target sequence:

(none)