Construct: shRNA TRCN0000422460

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178874.2-283s21c1
DNA Barcode:
GAAGCAAGCCTCCTGATTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmcc2 (68875)
Gene Description:
transmembrane and coiled-coil domains 2
Transcript:
RefSeq NM_178874.2 (NON-CURRENT)
Match location:
Position 283 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422460)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCAAGCCTCCTGATTTAA
Hairpin Sequence:
5'-CCGG-GAAGCAAGCCTCCTGATTTAA-CTCGAG-TTAAATCAGGAGGCTTGCTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCAAGCCTCCTGATTTAACTCGAGTTAAATCAGGAGGCTTGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCAAGCCTCCTGATTTAACTCGAGTTAAATCAGGAGGCTTGCTTC-3'

Other clones with same target sequence:

(none)