Construct: shRNA TRCN0000422538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030915.3-533s21c1
DNA Barcode:
TGTGGACTCCCATGGGTCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LBH (81606)
Gene Description:
LBH regulator of WNT signaling pathway
Transcript:
RefSeq NM_030915.3 (NON-CURRENT)
Match location:
Position 533 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGGACTCCCATGGGTCATA
Hairpin Sequence:
5'-CCGG-TGTGGACTCCCATGGGTCATA-CTCGAG-TATGACCCATGGGAGTCCACA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGGACTCCCATGGGTCATACTCGAGTATGACCCATGGGAGTCCACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGGACTCCCATGGGTCATACTCGAGTATGACCCATGGGAGTCCACA-3'

Other clones with same target sequence:

(none)