Construct: shRNA TRCN0000422542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183022.3-2334s21c1
DNA Barcode:
AGTCAGGGTCCTAGCCCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asic4 (241118)
Gene Description:
acid-sensing (proton-gated) ion channel family member 4
Transcript:
RefSeq NM_183022.3 (CURRENT)
Match location:
Position 2334 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCAGGGTCCTAGCCCTAAT
Hairpin Sequence:
5'-CCGG-AGTCAGGGTCCTAGCCCTAAT-CTCGAG-ATTAGGGCTAGGACCCTGACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCAGGGTCCTAGCCCTAATCTCGAGATTAGGGCTAGGACCCTGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCAGGGTCCTAGCCCTAATCTCGAGATTAGGGCTAGGACCCTGACT-3'

Other clones with same target sequence:

(none)