Construct: shRNA TRCN0000422573

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133445.2-4347s21c1
DNA Barcode:
CGTAACACAGAACGAGGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRIN3A (116443)
Gene Description:
glutamate ionotropic receptor NMDA type subunit 3A
Transcript:
RefSeq NM_133445.2 (NON-CURRENT)
Match location:
Position 4347 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422573)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTAACACAGAACGAGGTTAT
Hairpin Sequence:
5'-CCGG-CGTAACACAGAACGAGGTTAT-CTCGAG-ATAACCTCGTTCTGTGTTACG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTAACACAGAACGAGGTTATCTCGAGATAACCTCGTTCTGTGTTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTAACACAGAACGAGGTTATCTCGAGATAACCTCGTTCTGTGTTACG-3'

Other clones with same target sequence:

(none)