Construct: shRNA TRCN0000422615

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013264.3-1028s21c1
DNA Barcode:
ACCCTAATGTTATCAAGTTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDX25 (29118)
Gene Description:
DEAD-box helicase 25
Transcript:
RefSeq NM_013264.3 (NON-CURRENT)
Match location:
Position 1028 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422615)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCTAATGTTATCAAGTTAC
Hairpin Sequence:
5'-CCGG-ACCCTAATGTTATCAAGTTAC-CTCGAG-GTAACTTGATAACATTAGGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCTAATGTTATCAAGTTACCTCGAGGTAACTTGATAACATTAGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCTAATGTTATCAAGTTACCTCGAGGTAACTTGATAACATTAGGGT-3'

Other clones with same target sequence:

(none)