Construct: shRNA TRCN0000422622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178276.4-482s21c1
DNA Barcode:
GTAGAGCTCATATTCACAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SERINC5 (256987)
Gene Description:
serine incorporator 5
Transcript:
RefSeq NM_178276.4 (NON-CURRENT)
Match location:
Position 482 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGAGCTCATATTCACAATG
Hairpin Sequence:
5'-CCGG-GTAGAGCTCATATTCACAATG-CTCGAG-CATTGTGAATATGAGCTCTAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGAGCTCATATTCACAATGCTCGAGCATTGTGAATATGAGCTCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGAGCTCATATTCACAATGCTCGAGCATTGTGAATATGAGCTCTAC-3'

Other clones with same target sequence:

(none)