Construct: shRNA TRCN0000422626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172987.2-1252s21c1
DNA Barcode:
TAATTGTTTGTGCATCTAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nkain3 (269513)
Gene Description:
Na+/K+ transporting ATPase interacting 3
Transcript:
RefSeq NM_172987.2 (CURRENT)
Match location:
Position 1252 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAATTGTTTGTGCATCTAAAC
Hairpin Sequence:
5'-CCGG-TAATTGTTTGTGCATCTAAAC-CTCGAG-GTTTAGATGCACAAACAATTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAATTGTTTGTGCATCTAAACCTCGAGGTTTAGATGCACAAACAATTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAATTGTTTGTGCATCTAAACCTCGAGGTTTAGATGCACAAACAATTA-3'

Other clones with same target sequence:

(none)