Construct: shRNA TRCN0000422652

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017754.3-4341s21c1
DNA Barcode:
ACCATCAGCTGAAGTACTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UHRF1BP1 (54887)
Gene Description:
UHRF1 binding protein 1
Transcript:
RefSeq NM_017754.3 (NON-CURRENT)
Match location:
Position 4341 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422652)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCATCAGCTGAAGTACTTAA
Hairpin Sequence:
5'-CCGG-ACCATCAGCTGAAGTACTTAA-CTCGAG-TTAAGTACTTCAGCTGATGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCATCAGCTGAAGTACTTAACTCGAGTTAAGTACTTCAGCTGATGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCATCAGCTGAAGTACTTAACTCGAGTTAAGTACTTCAGCTGATGGT-3'

Other clones with same target sequence:

(none)