Construct: shRNA TRCN0000422654

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182894.2-727s21c1
DNA Barcode:
AGCAGTGTCATGGCGGAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VSX2 (338917)
Gene Description:
visual system homeobox 2
Transcript:
RefSeq NM_182894.2 (NON-CURRENT)
Match location:
Position 727 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422654)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGTGTCATGGCGGAGTAT
Hairpin Sequence:
5'-CCGG-AGCAGTGTCATGGCGGAGTAT-CTCGAG-ATACTCCGCCATGACACTGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGTGTCATGGCGGAGTATCTCGAGATACTCCGCCATGACACTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGTGTCATGGCGGAGTATCTCGAGATACTCCGCCATGACACTGCT-3'

Other clones with same target sequence:

(none)