Construct: shRNA TRCN0000422704

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198619.2-1992s21c1
DNA Barcode:
CATGTGGGAAATTGATCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp933 (242747)
Gene Description:
zinc finger protein 933
Transcript:
RefSeq NM_198619.2 (CURRENT)
Match location:
Position 1992 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422704)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGTGGGAAATTGATCTATA
Hairpin Sequence:
5'-CCGG-CATGTGGGAAATTGATCTATA-CTCGAG-TATAGATCAATTTCCCACATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGTGGGAAATTGATCTATACTCGAGTATAGATCAATTTCCCACATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGTGGGAAATTGATCTATACTCGAGTATAGATCAATTTCCCACATG-3'

Other clones with same target sequence:

(none)