Construct: shRNA TRCN0000422736

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008342.2-1124s21c1
DNA Barcode:
AGACCAACACTGAGCTCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Igfbp2 (16008)
Gene Description:
insulin-like growth factor binding protein 2
Transcript:
RefSeq NM_008342.2 (NON-CURRENT)
Match location:
Position 1124 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422736)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACCAACACTGAGCTCAGAA
Hairpin Sequence:
5'-CCGG-AGACCAACACTGAGCTCAGAA-CTCGAG-TTCTGAGCTCAGTGTTGGTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACCAACACTGAGCTCAGAACTCGAGTTCTGAGCTCAGTGTTGGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACCAACACTGAGCTCAGAACTCGAGTTCTGAGCTCAGTGTTGGTCT-3'

Other clones with same target sequence:

(none)