Construct: shRNA TRCN0000422788

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000273.2-971s21c1
DNA Barcode:
GATATCAATGGAGGTTCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPR143 (4935)
Gene Description:
G protein-coupled receptor 143
Transcript:
RefSeq NM_000273.2 (NON-CURRENT)
Match location:
Position 971 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422788)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATATCAATGGAGGTTCTTTG
Hairpin Sequence:
5'-CCGG-GATATCAATGGAGGTTCTTTG-CTCGAG-CAAAGAACCTCCATTGATATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATATCAATGGAGGTTCTTTGCTCGAGCAAAGAACCTCCATTGATATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATATCAATGGAGGTTCTTTGCTCGAGCAAAGAACCTCCATTGATATC-3'

Other clones with same target sequence:

(none)