Construct: shRNA TRCN0000422807

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024318.2-1735s21c1
DNA Barcode:
ACCTTACTTCCAATCTATGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LILRA6 (79168)
Gene Description:
leukocyte immunoglobulin like receptor A6
Transcript:
RefSeq NM_024318.2 (NON-CURRENT)
Match location:
Position 1735 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422807)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTTACTTCCAATCTATGTT
Hairpin Sequence:
5'-CCGG-ACCTTACTTCCAATCTATGTT-CTCGAG-AACATAGATTGGAAGTAAGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTTACTTCCAATCTATGTTCTCGAGAACATAGATTGGAAGTAAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTTACTTCCAATCTATGTTCTCGAGAACATAGATTGGAAGTAAGGT-3'

Other clones with same target sequence:

(none)