Construct: shRNA TRCN0000422859

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031955.4-1060s21c1
DNA Barcode:
GCATAAGCAAGCTCATCAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPATA16 (83893)
Gene Description:
spermatogenesis associated 16
Transcript:
RefSeq NM_031955.4 (NON-CURRENT)
Match location:
Position 1060 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422859)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATAAGCAAGCTCATCAAAC
Hairpin Sequence:
5'-CCGG-GCATAAGCAAGCTCATCAAAC-CTCGAG-GTTTGATGAGCTTGCTTATGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATAAGCAAGCTCATCAAACCTCGAGGTTTGATGAGCTTGCTTATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATAAGCAAGCTCATCAAACCTCGAGGTTTGATGAGCTTGCTTATGC-3'

Other clones with same target sequence:

(none)