Construct: shRNA TRCN0000422925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001045.4-2349s21c1
DNA Barcode:
GGACATTTAAAGAGCGTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC6A4 (6532)
Gene Description:
solute carrier family 6 member 4
Transcript:
RefSeq NM_001045.4 (NON-CURRENT)
Match location:
Position 2349 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACATTTAAAGAGCGTATTA
Hairpin Sequence:
5'-CCGG-GGACATTTAAAGAGCGTATTA-CTCGAG-TAATACGCTCTTTAAATGTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACATTTAAAGAGCGTATTACTCGAGTAATACGCTCTTTAAATGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACATTTAAAGAGCGTATTACTCGAGTAATACGCTCTTTAAATGTCC-3'

Other clones with same target sequence:

(none)