Construct: shRNA TRCN0000422974
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_000306.2-677s21c1
- DNA Barcode:
- CAAACTGAAAGCAATATTATC
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- POU1F1 (5449)
- Gene Description:
- POU class 1 homeobox 1
- Transcript:
- RefSeq NM_000306.2 (NON-CURRENT)
- Match location:
- Position 677 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000422974)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- CAAACTGAAAGCAATATTATC
- Hairpin Sequence:
- 5'-CCGG-CAAACTGAAAGCAATATTATC-CTCGAG-GATAATATTGCTTTCAGTTTG-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAAACTGAAAGCAATATTATCCTCGAGGATAATATTGCTTTCAGTTTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAAACTGAAAGCAATATTATCCTCGAGGATAATATTGCTTTCAGTTTG-3'